Protocols

IMMUNOFLUORESCENCE

 

• 4% PFA fixation and permeabilisation step
• Primary antibodies mix application
• Overnight incubation
• Rinse with PBS (next day)
• Secondary antibodies mix application – incubation for 45 minutes at room temperature.
• Rinse with PBS (3 times)
• Coverslip with immunofluorescent mounting media with DAPI
• Leave to dry by applying some pressure
• Once dry seal with nail varnish

 

Picture description: Triple stain (CD158, GFAP, NKp46) in human diseased brain demonstrating presence of CD158 and NKp46 on astrocytes.

This picture has been contributed by Dr Pascal Durrenberger.