Professor M Seabra

Publications


Journals

  • Hendrix A; Braems G; Bracke M; Seabra MC; Gahl WA; De Wever O; Westbroek W. (1 Aug 2010). The secretory small GTPase Rab27B as a marker for breast cancer progression. ONCOTARGET. 1:304-308.
  • Diekmann Y; Seixas E; Gouw M; Tavares-Cadete F; Seabra MC; Pereira-Leal JB. (Oct 2011). Thousands of rab GTPases for the cell biologist. PLoS Comput Biol. 7:e1002217. DOI.
  • Hume AN; Seabra MC. (Oct 2011). Melanosomes on the move: a model to understand organelle dynamics. Biochem Soc Trans. 39:1191-1196. DOI.
  • Taylor A; Mules EH; Seabra MC; Helfrich MH; Rogers MJ; Coxon FP. Impaired prenylation of Rab GTPases in the gunmetal mouse causes defects in bone cell function. Small Gtpases. 2:131-142. DOI.
  • Tarafder AK; Wasmeier C; Figueiredo AC; Booth AE; Orihara A; Ramalho JS; Hume AN; Seabra MC. (Aug 2011). Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding. Traffic. 12:1056-1066. DOI.
  • Coxon FP; Taylor A; Stewart CA; Baron R; Seabra MC; Ebetino FH; Rogers MJ. (Jul 2011). The gunmetal mouse reveals Rab geranylgeranyl transferase to be the major molecular target of phosphonocarboxylate analogues of bisphosphonates. Bone. 49:111-121. DOI.
  • Hendrix A; Maynard D; Pauwels P; Braems G; Denys H; Van den Broecke R; Lambert J; Van Belle S; et alCocquyt V; Gespach C; Bracke M; Seabra MC; Gahl WA; De Wever O; Westbroek W. (16 Jun 2010). Effect of the secretory small GTPase Rab27B on breast cancer growth, invasion, and metastasis. J Natl Cancer Inst. 102:866-880. DOI.
  • Ostrowski M; Carmo NB; Krumeich S; Fanget I; Raposo G; Savina A; Moita CF; Schauer K; et alHume AN; Freitas RP; Goud B; Benaroch P; Hacohen N; Fukuda M; Desnos C; Seabra MC; Darchen F; Amigorena S; Moita LF; Thery C. (Jan 2010). Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 12:19-30. DOI.
  • Ramalho JS; Lopes VS; Tarafder AK; Seabra MC; Hume AN. (Aug 2009). Myrip uses distinct domains in the cellular activation of myosin VA and myosin VIIA in melanosome transport. Pigment Cell Melanoma Res. 22:461-473. DOI.
  • Baron RA; Tavaré R; Figueiredo AC; Błazewska KM; Kashemirov BA; McKenna CE; Ebetino FH; Taylor A; et alRogers MJ; Coxon FP; Seabra MC. (13 Mar 2009). Phosphonocarboxylates inhibit the second geranylgeranyl addition by Rab geranylgeranyl transferase. J Biol Chem. 284:6861-6868. DOI.
  • Hume AN; Buttgereit J; Al-Awadhi AM; Al-Suwaidi SS; John A; Bader M; Seabra MC; Al-Gazali L; et alAli BR. (15 Jan 2009). Defective cellular trafficking of missense NPR-B mutants is the major mechanism underlying acromesomelic dysplasia-type Maroteaux. Hum Mol Genet. 18:267-277. DOI.
  • Figueiredo AC; Wasmeier C; Tarafder AK; Ramalho JS; Baron RA; Seabra MC. (22 Aug 2008). Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes. J Biol Chem. 283:23209-23216. DOI.
  • Baron RA; Seabra MC. (1 Oct 2008). Rab geranylgeranylation occurs preferentially via the pre-formed REP-RGGT complex and is regulated by geranylgeranyl pyrophosphate. Biochem J. 415:67-75. DOI.
  • Lopes VS; Wasmeier C; Seabra MC; Futter CE. (Oct 2007). Melanosome maturation defect in Rab38-deficient retinal pigment epithelium results in instability of immature melanosomes during transient melanogenesis. Mol Biol Cell. 18:3914-3927. DOI.
  • Futter CE; Lopes VS; Wavre ST; Seabra MC. (1 Apr 2008). Melanosome biogenesis, transport and function in retinal pigment epithelial cells. PIGMENT CELL & MELANOMA RESEARCH. 21:255-255. Author weblink.
  • Frasa MAM; Maximiano FC; Smolarczyk K; Francis RE; Betson ME; Lozano E; Goldenring J; Seabra MC; et alRak A; Ahmadian MR; Braga VMM. (9 Feb 2010). Armus Is a Rac1 Effector that Inactivates Rab7 and Regulates E-Cadherin Degradation. CURRENT BIOLOGY. 20:198-208. Author weblink DOI.
  • Coxon FP; Stewart CA; Tavare R; Baron R; Taylor A; Marma MS; Blazewska KM; Kashemirov BA; et alEbetino FH; Seabra MC; Rogers MJ; McKenna CE. (1 Jan 2008). Inhibition of Rab geranylgeranyl transferase and Rab prenylation by phosphonocarboxylate drugs. CALCIFIED TISSUE INTERNATIONAL. 82:S127-S127.
  • Nightingale TD; Pattni K; Hume AN; Seabra MC; Cutler DF. (24 Mar 2011). Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells (vol 113, pg 5010, 2009). BLOOD. 117:3476-3476. DOI.
  • Nightingale TD; Pattni K; Hume AN; Seabra MC; Cutler DF. (14 May 2009). Rab27a and MyRIP regulate the amount and multimeric state of VWF released from endothelial cells. BLOOD. 113:5010-5018. DOI.
  • Pulido IR; Nightingale TD; Darchen F; Seabra MC; Cutler DF; Gerke V. (1 Oct 2011). Myosin Va Acts in Concert with Rab27a and MyRIP to Regulate Acute Von-Willebrand Factor Release from Endothelial Cells. TRAFFIC. 12:1371-1382. DOI.
  • Mizuno K; Tolmachova T; Ushakov DS; Romao M; Abrink M; Ferenczi MA; Raposo G; Seabra MC. (Jul 2007). Rab27b regulates mast cell granule dynamics and secretion. Traffic. 8:883-892. DOI.
  • Hume AN; Ushakov DS; Tarafder AK; Ferenczi MA; Seabra MC. (1 Sep 2007). Rab27a and MyoVa are the primary MIph interactors regulating melanosome transport in melanocytes. JOURNAL OF CELL SCIENCE. 120:3111-3122. Author weblink DOI.
  • Wasmeier C; Hume AN; Bolasco G; Seabra MC. (15 Dec 2008). Melanosomes at a glance. J Cell Sci. 121:3995-3999. DOI.
  • Moosajee M; Tulloch M; Baron RA; Gregory-Evans CY; Pereira-Leal JB; Seabra MC. (Jun 2009). Single choroideremia gene in nonmammalian vertebrates explains early embryonic lethality of the zebrafish model of choroideremia. Invest Ophthalmol Vis Sci. 50:3009-3016. DOI.
  • Johnson JL; Brzezinska AA; Tolmachova T; Munafo DB; Ellis BA; Seabra MC; Hong H; Catz SD. (Apr 2010). Rab27a and Rab27b regulate neutrophil azurophilic granule exocytosis and NADPH oxidase activity by independent mechanisms. Traffic. 11:533-547. DOI.
  • Berry AF; Heal WP; Tarafder AK; Tolmachova T; Baron RA; Seabra MC; Tate EW. (12 Apr 2010). Rapid multilabel detection of geranylgeranylated proteins by using bioorthogonal ligation chemistry. Chembiochem. 11:771-773. DOI.
  • Tolmachova T; Wavre-Shapton ST; Barnard AR; MacLaren RE; Futter CE; Seabra MC. (Oct 2010). Retinal pigment epithelium defects accelerate photoreceptor degeneration in cell type-specific knockout mouse models of choroideremia. Invest Ophthalmol Vis Sci. 51:4913-4920. DOI.
  • Anderson KE; Chessa TA; Davidson K; Henderson RB; Walker S; Tolmachova T; Grys K; Rausch O; et alSeabra MC; Tybulewicz VL; Stephens LR; Hawkins PT. (2 Dec 2010). PtdIns3P and Rac direct the assembly of the NADPH oxidase on a novel, pre-phagosomal compartment during FcR-mediated phagocytosis in primary mouse neutrophils. Blood. 116:4978-4989. DOI.
  • Bolasco G; Tracey-White DC; Tolmachova T; Thorley AJ; Tetley TD; Seabra MC; Hume AN. (Mar 2011). Loss of Rab27 function results in abnormal lung epithelium structure in mice. Am J Physiol Cell Physiol. 300:C466-C476. DOI.
  • Chiang L; Ngo J; Schechter JE; Karvar S; Tolmachova T; Seabra MC; Hume AN; Hamm-Alvarez SF. (Aug 2011). Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland. Am J Physiol Cell Physiol. 301:C507-C521. DOI.
  • Hume AN; Wilson MS; Ushakov DS; Ferenczi MA; Seabra MC. (Dec 2011). Semi-automated analysis of organelle movement and membrane content: understanding rab-motor complex transport function. Traffic. 12:1686-1701. DOI.
  • Singh RK; Liao W; Tracey-White D; Recchi C; Tolmachova T; Rankin SM; Hume AN; Seabra MC. (1 Apr 2012). Rab27a-mediated protease release regulates neutrophil recruitment by allowing uropod detachment. J Cell Sci. 125:1652-1656. DOI.
  • Tolmachova T; Tolmachov OE; Wavre-Shapton ST; Tracey-White D; Futter CE; Seabra MC. (Mar 2012). CHM/REP1 cDNA delivery by lentiviral vectors provides functional expression of the transgene in the retinal pigment epithelium of choroideremia mice. J Gene Med. 14:158-168. DOI.
  • Blazewska KM; Ni F; Haiges R; Kashemirov BA; Coxon FP; Stewart CA; Baron R; Rogers MJ; et alSeabra MC; Ebetino FH; McKenna CE. (1 Oct 2011). Synthesis, stereochemistry and SAR of a series of minodronate analogues as RGGT inhibitors. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. 46:4820-4826. Author weblink DOI.
  • Ali BR; Nouvel I; Leung KF; Hume AN; Seabra MC. (18 Jun 2010). A novel statin-mediated "prenylation block-and-release" assay provides insight into the membrane targeting mechanisms of small GTPases. Biochem Biophys Res Commun. 397:34-41. DOI.
  • McKenna CE; Kashemirov BA; Błazewska KM; Mallard-Favier I; Stewart CA; Rojas J; Lundy MW; Ebetino FH; et alBaron RA; Dunford JE; Kirsten ML; Seabra MC; Bala JL; Marma MS; Rogers MJ; Coxon FP. (13 May 2010). Synthesis, chiral high performance liquid chromatographic resolution and enantiospecific activity of a potent new geranylgeranyl transferase inhibitor, 2-hydroxy-3-imidazo[1,2-a]pyridin-3-yl-2-phosphonopropionic acid. J Med Chem. 53:3454-3464. DOI.
  • Moosajee M; Gregory-Evans K; Ellis CD; Seabra MC; Gregory-Evans CY. (15 Dec 2008). Translational bypass of nonsense mutations in zebrafish rep1, pax2.1 and lamb1 highlights a viable therapeutic option for untreatable genetic eye disease. Hum Mol Genet. 17:3987-4000. DOI.
  • Lopes VS; Ramalho JS; Owen DM; Karl MO; Strauss O; Futter CE; Seabra MC. (May 2007). The ternary Rab27a-Myrip-Myosin VIIa complex regulates melanosome motility in the retinal pigment epithelium. Traffic. 8:486-499. DOI.
  • Tolmachova T; Abrink M; Futter CE; Authi KS; Seabra MC. (3 Apr 2007). Rab27b regulates number and secretion of platelet dense granules. Proc Natl Acad Sci U S A. 104:5872-5877. DOI.
  • Jancic C; Savina A; Wasmeier C; Tolmachova T; El-Benna J; Dang PM; Pascolo S; Gougerot-Pocidalo MA; et alRaposo G; Seabra MC; Amigorena S. (Apr 2007). Rab27a regulates phagosomal pH and NADPH oxidase recruitment to dendritic cell phagosomes. Nat Cell Biol. 9:367-378. DOI.
  • Leung KF; Baron R; Ali BR; Magee AI; Seabra MC. (12 Jan 2007). Rab GTPases containing a CAAX motif are processed post-geranylgeranylation by proteolysis and methylation. J Biol Chem. 282:1487-1497. DOI.
  • Hume AN; Ushakov DS; Tarafder AK; Ferenczi MF; Seabra MC. (2007). Rab27a and MyoVa are the primary Mlph interactors regulating melanosome transport in melanocytes. J Cell Sci. 120:3111-3122.
  • Mizuno K; Tolmachova T; Ushakov DS; Romao M; Abrink M; Ferenczi MF; Raposo G; Seabra MC. (2007). Rab27b regulates mast cell granule dynamics and secretion. Traffic. 8:883-892.
  • Onfelt B; Nedvetzki S; Benninger RK; Purbhoo MA; Sowinski S; Hume AN; Seabra MC; Neil MA; et alFrench PM; Davis DM. (15 Dec 2006). Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria. J Immunol. 177:8476-8483.
  • Hume AN; Tarafder AK; Ramalho JS; Sviderskaya EV; Seabra MC. (Nov 2006). A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes. Mol Biol Cell. 17:4720-4735. DOI.
  • Wasmeier C; Romao M; Plowright L; Bennett DC; Raposo G; Seabra MC. (23 Oct 2006). Rab38 and Rab32 control post-Golgi trafficking of melanogenic enzymes. J Cell Biol. 175:271-281. DOI.
  • Savina A; Jancic C; Hugues S; Guermonprez P; Vargas P; Moura IC; Lennon-Duménil AM; Seabra MC; et alRaposo G; Amigorena S. (14 Jul 2006). NOX2 controls phagosomal pH to regulate antigen processing during crosspresentation by dendritic cells. Cell. 126:205-218. DOI.
  • Leung KF; Baron R; Seabra MC. (Mar 2006). Thematic review series: lipid posttranslational modifications. geranylgeranylation of Rab GTPases. J Lipid Res. 47:467-475. DOI.
  • Tolmachova T; Anders R; Abrink M; Bugeon L; Dallman MJ; Futter CE; Ramalho JS; Tonagel F; et alTanimoto N; Seeliger MW; Huxley C; Seabra MC. (Feb 2006). Independent degeneration of photoreceptors and retinal pigment epithelium in conditional knockout mouse models of choroideremia. J Clin Invest. 116:386-394. DOI.
  • Johnson JL; Ellis BA; Noack D; Seabra MC; Catz SD. (1 Nov 2005). The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase. BIOCHEMICAL JOURNAL. 391:699-710. Author weblink DOI.
  • Chen Y; Bellamy WP; Seabra MC; Field MC; Ali BR. (1 Sep 2005). ER-associated protein degradation is a common mechanism underpinning numerous monogenic diseases including Robinow syndrome. HUMAN MOLECULAR GENETICS. 14:2559-2569. Author weblink DOI.
  • Coxon FP; Ebetino FH; Mules EH; Seabra MC; McKenna CE; Rogers MJ. (1 Sep 2005). Phosphonocarboxylate inhibitors of Rab geranylgeranyl transferase disrupt the prenylation and membrane localization of Rab proteins in osteoclasts in vitro and in vivo. BONE. 37:349-358. Author weblink DOI.
  • Ali BR; Seabra MC. (1 Aug 2005). Targeting of Rab GTPases to cellular membranes. BIOCHEMICAL SOCIETY TRANSACTIONS. 33:652-656. Author weblink.
  • Magee T; Seabra MC. (Apr 2005). Fatty acylation and prenylation of proteins: what's hot in fat. Curr Opin Cell Biol. 17:190-196. DOI.
  • Ali BR; Wasmeier C; Lamoreux L; Strom M; Seabra MC. (15 Dec 2004). Multiple regions contribute to membrane targeting of Rab GTPases. J Cell Sci. 117:6401-6412. DOI.
  • Westbroek W; Lambert J; De Schepper S; Kleta R; Van Den Bossche K; Seabra MC; Huizing M; Mommaas M; et alNaeyaert JM. (Oct 2004). Rab27b is up-regulated in human Griscelli syndrome type II melanocytes and linked to the actin cytoskeleton via exon F-Myosin Va transcripts. Pigment Cell Res. 17:498-505. DOI.
  • Seabra MC; Wasmeier C. (Aug 2004). Controlling the location and activation of Rab GTPases. Curr Opin Cell Biol. 16:451-457. DOI.
  • Seabra MC; Coudrier E. (Jun 2004). Rab GTPases and myosin motors in organelle motility. Traffic. 5:393-399. DOI.
  • Futter CE; Ramalho JS; Jaissle GB; Seeliger MW; Seabra MC. (May 2004). The role of Rab27a in the regulation of melanosome distribution within retinal pigment epithelial cells. Mol Biol Cell. 15:2264-2275. DOI.
  • Barral DC; Seabra MC. (Apr 2004). The melanosome as a model to study organelle motility in mammals. Pigment Cell Res. 17:111-118. DOI.
  • Ramalho JS; Gregory-Evans K; Huxley C; Seabra MC. (Mar 2004). Mouse genetic corneal disease resulting from transgenic insertional mutagenesis. Br J Ophthalmol. 88:428-432.
  • Tolmachova T; Anders R; Stinchcombe J; Bossi G; Griffiths GM; Huxley C; Seabra MC. (Jan 2004). A general role for Rab27a in secretory cells. Mol Biol Cell. 15:332-344. DOI.
  • Tiwari S; Italiano JE; Barral DC; Mules EH; Novak EK; Swank RT; Seabra MC; Shivdasani RA. (1 Dec 2003). A role for Rab27b in NF-E2-dependent pathways of platelet formation. Blood. 102:3970-3979. DOI.
  • Magee AI; Seabra MC. (1 Dec 2003). Are prenyl groups on proteins sticky fingers or greasy handles?. Biochem J. 376:e3-e4. DOI.
  • Larijani B; Hume AN; Tarafder AK; Seabra MC. (21 Nov 2003). Multiple factors contribute to inefficient prenylation of Rab27a in Rab prenylation diseases. J Biol Chem. 278:46798-46804. DOI.
  • Hannah MJ; Hume AN; Arribas M; Williams R; Hewlett LJ; Seabra MC; Cutler DF. (1 Oct 2003). Weibel-Palade bodies recruit Rab27 by a content-driven, maturation-dependent mechanism that is independent of cell type. J Cell Sci. 116:3939-3948. DOI.
  • Gomes AQ; Ali BR; Ramalho JS; Godfrey RF; Barral DC; Hume AN; Seabra MC. (May 2003). Membrane targeting of Rab GTPases is influenced by the prenylation motif. Mol Biol Cell. 14:1882-1899. DOI.
  • Anand V; Barral DC; Zeng Y; Brunsmann F; Maguire AM; Seabra MC; Bennett J. (Apr 2003). Gene therapy for choroideremia: in vitro rescue mediated by recombinant adenovirus. Vision Res. 43:919-926.
  • Pereira-Leal JB; Strom M; Godfrey RF; Seabra MC. (31 Jan 2003). Structural determinants of Rab and Rab Escort Protein interaction: Rab family motifs define a conserved binding surface. Biochem Biophys Res Commun. 301:92-97.
  • Ramalho JS; Anders R; Jaissle GB; Seeliger MW; Huxley C; Seabra MC. (28 Oct 2002). Rapid degradation of dominant-negative Rab27 proteins in vivo precludes their use in transgenic mouse models. BMC Cell Biol. 3:26.
  • Barral DC; Ramalho JS; Anders R; Hume AN; Knapton HJ; Tolmachova T; Collinson LM; Goulding D; et alAuthi KS; Seabra MC. (1 Oct 2002). Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome (vol 110, pg 247, 2002). JOURNAL OF CLINICAL INVESTIGATION. 110:1213-1213. Author weblink DOI.
  • Strom M; Hume AN; Tarafder AK; Barkagianni E; Seabra MC. (12 Jul 2002). A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport. J Biol Chem. 277:25423-25430. DOI.
  • Barral DC; Ramalho JS; Anders R; Hume AN; Knapton HJ; Tolmachova T; Collinson LM; Goulding D; et alAuthi KS; Seabra MC. (Jul 2002). Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome. J Clin Invest. 110:247-257. DOI.
  • Hume AN; Collinson LM; Hopkins CR; Strom M; Barral DC; Bossi G; Griffiths GM; Seabra MC. (Mar 2002). The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes. Traffic. 3:193-202.
  • Pereira-Leal JB; Gomes AQ; Seabra MC. (2002). Analysis and preparation of stable complexes between Rab GTPases, Rab escort protein, and Rab geranylgeranyl transferase. Methods Mol Biol. 189:157-165. DOI.
  • Seabra MC; Mules EH; Hume AN. (Jan 2002). Rab GTPases, intracellular traffic and disease. Trends Mol Med. 8:23-30.
  • Coxon FP; Helfrich MH; Larijani B; Muzylak M; Dunford JE; Marshall D; McKinnon AD; Nesbitt SA; et alHorton MA; Seabra MC; Ebetino FH; Rogers MJ. (21 Dec 2001). Identification of a novel phosphonocarboxylate inhibitor of Rab geranylgeranyl transferase that specifically prevents Rab prenylation in osteoclasts and macrophages. J Biol Chem. 276:48213-48222. DOI.
  • Pereira-Leal JB; Seabra MC. (2 Nov 2001). Evolution of the Rab family of small GTP-binding proteins. J Mol Biol. 313:889-901. DOI.
  • Marks MS; Seabra MC. (Oct 2001). The melanosome: membrane dynamics in black and white. Nat Rev Mol Cell Biol. 2:738-748. DOI.
  • Pereira-Leal JB; Hume AN; Seabra MC. (8 Jun 2001). Prenylation of Rab GTPases: molecular mechanisms and involvement in genetic disease. FEBS Lett. 498:197-200.
  • Syed N; Smith JE; John SK; Seabra MC; Aguirre GD; Milam AH. (Apr 2001). Evaluation of retinal photoreceptors and pigment epithelium in a female carrier of choroideremia. Ophthalmology. 108:711-720.
  • Bergo MO; Leung GK; Ambroziak P; Otto JC; Casey PJ; Gomes AQ; Seabra MC; Young SG. (23 Feb 2001). Isoprenylcysteine carboxyl methyltransferase deficiency in mice. J Biol Chem. 276:5841-5845. DOI.
  • Stinchcombe JC; Barral DC; Mules EH; Booth S; Hume AN; Machesky LM; Seabra MC; Griffiths GM. (19 Feb 2001). Rab27a is required for regulated secretion in cytotoxic T lymphocytes. J Cell Biol. 152:825-834.
  • Hume AN; Collinson LM; Rapak A; Gomes AQ; Hopkins CR; Seabra MC. (19 Feb 2001). Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J Cell Biol. 152:795-808.
  • Ramalho JS; Tolmachova T; Hume AN; McGuigan A; Gregory-Evans CY; Huxley C; Seabra MC. (2001). Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene. BMC Genet. 2:2.
  • Pereira-Leal JB; Seabra MC. (25 Aug 2000). The mammalian Rab family of small GTPases: definition of family and subfamily sequence motifs suggests a mechanism for functional specificity in the Ras superfamily. J Mol Biol. 301:1077-1087. DOI.
  • Detter JC; Zhang Q; Mules EH; Novak EK; Mishra VS; Li W; McMurtrie EB; Tchernev VT; et alWallace MR; Seabra MC; Swank RT; Kingsmore SF. (11 Apr 2000). Rab geranylgeranyl transferase alpha mutation in the gunmetal mouse reduces Rab prenylation and platelet synthesis. Proc Natl Acad Sci U S A. 97:4144-4149. DOI.
  • Zhang H; Seabra MC; Deisenhofer J. (15 Mar 2000). Crystal structure of Rab geranylgeranyltransferase at 2.0 A resolution. Structure. 8:241-251.
  • Tolmachova T; Ramalho JS; Anant JS; Schultz RA; Huxley CM; Seabra MC. (18 Oct 1999). Cloning, mapping and characterization of the human RAB27A gene. Gene. 239:109-116.
  • Desnoyers L; Seabra MC. (13 Oct 1998). Single prenyl-binding site on protein prenyl transferases. Proc Natl Acad Sci U S A. 95:12266-12270.
  • Anant JS; Desnoyers L; Machius M; Demeler B; Hansen JC; Westover KD; Deisenhofer J; Seabra MC. (8 Sep 1998). Mechanism of Rab geranylgeranylation: formation of the catalytic ternary complex. Biochemistry. 37:12559-12568. DOI.
  • MacDonald IM; Mah DY; Ho YK; Lewis RA; Seabra MC. (Sep 1998). A practical diagnostic test for choroideremia. Ophthalmology. 105:1637-1640. DOI.
  • Seabra MC. (Mar 1998). Membrane association and targeting of prenylated Ras-like GTPases. Cell Signal. 10:167-172.
  • Seabra MC; James GL. (1998). Prenylation assays for small GTPases. Methods Mol Biol. 84:251-260. DOI.
  • van Bokhoven H; Rawson RB; Merkx GF; Cremers FP; Seabra MC. (1 Dec 1996). cDNA cloning and chromosomal localization of the genes encoding the alpha- and beta-subunits of human Rab geranylgeranyl transferase: the 3' end of the alpha-subunit gene overlaps with the transglutaminase 1 gene promoter. Genomics. 38:133-140.
  • Rybin V; Ullrich O; Rubino M; Alexandrov K; Simon I; Seabra MC; Goody R; Zerial M. (19 Sep 1996). GTPase activity of Rab5 acts as a timer for endocytic membrane fusion. Nature. 383:266-269. DOI.
  • Desnoyers L; Anant JS; Seabra MC. (Aug 1996). Geranylgeranylation of Rab proteins. Biochem Soc Trans. 24:699-703.
  • Seabra MC. (14 Jun 1996). Nucleotide dependence of Rab geranylgeranylation. Rab escort protein interacts preferentially with GDP-bound Rab. J Biol Chem. 271:14398-14404.
  • Seabra MC. (Jun 1996). New insights into the pathogenesis of choroideremia: a tale of two REPs. Ophthalmic Genet. 17:43-46.
  • Casey PJ; Seabra MC. (8 Mar 1996). Protein prenyltransferases. J Biol Chem. 271:5289-5292.
  • Wyne KL; Pathak K; Seabra MC; Hobbs HH. (Mar 1996). Expression of the VLDL receptor in endothelial cells. Arterioscler Thromb Vasc Biol. 16:407-415.
  • Shen F; Seabra MC. (16 Feb 1996). Mechanism of digeranylgeranylation of Rab proteins. Formation of a complex between monogeranylgeranyl-Rab and Rab escort protein. J Biol Chem. 271:3692-3698.
  • Mooser V; Seabra MC; Abedin M; Landschulz KT; Marcovina S; Hobbs HH. (1 Feb 1996). Apolipoprotein(a) kringle 4-containing fragments in human urine. Relationship to plasma levels of lipoprotein(a). J Clin Invest. 97:858-864. DOI.
  • Seabra MC; Ho YK; Anant JS. (13 Oct 1995). Deficient geranylgeranylation of Ram/Rab27 in choroideremia. J Biol Chem. 270:24420-24427.
  • Armstrong SA; Brown MS; Goldstein JL; Seabra MC. (1995). Preparation of recombinant Rab geranylgeranyltransferase and Rab escort proteins. Methods Enzymol. 257:30-41.
  • Farnsworth CC; Seabra MC; Ericsson LH; Gelb MH; Glomset JA. (6 Dec 1994). Rab geranylgeranyl transferase catalyzes the geranylgeranylation of adjacent cysteines in the small GTPases Rab1A, Rab3A, and Rab5A. Proc Natl Acad Sci U S A. 91:11963-11967.
  • Alexandrov K; Horiuchi H; Steele-Mortimer O; Seabra MC; Zerial M. (15 Nov 1994). Rab escort protein-1 is a multifunctional protein that accompanies newly prenylated rab proteins to their target membranes. EMBO J. 13:5262-5273.
  • Smeland TE; Seabra MC; Goldstein JL; Brown MS. (25 Oct 1994). Geranylgeranylated Rab proteins terminating in Cys-Ala-Cys, but not Cys-Cys, are carboxyl-methylated by bovine brain membranes in vitro. Proc Natl Acad Sci U S A. 91:10712-10716.
  • Cremers FP; Armstrong SA; Seabra MC; Brown MS; Goldstein JL. (21 Jan 1994). REP-2, a Rab escort protein encoded by the choroideremia-like gene. J Biol Chem. 269:2111-2117.
  • Andres DA; Seabra MC; Brown MS; Armstrong SA; Smeland TE; Cremers FP; Goldstein JL. (18 Jun 1993). cDNA cloning of component A of Rab geranylgeranyl transferase and demonstration of its role as a Rab escort protein. Cell. 73:1091-1099.
  • Armstrong SA; Seabra MC; Südhof TC; Goldstein JL; Brown MS. (5 Jun 1993). cDNA cloning and expression of the alpha and beta subunits of rat Rab geranylgeranyl transferase. J Biol Chem. 268:12221-12229.
  • Seabra MC; Brown MS; Goldstein JL. (15 Jan 1993). Retinal degeneration in choroideremia: deficiency of rab geranylgeranyl transferase. Science. 259:377-381.
  • Seabra MC; Brown MS; Slaughter CA; Südhof TC; Goldstein JL. (18 Sep 1992). Purification of component A of Rab geranylgeranyl transferase: possible identity with the choroideremia gene product. Cell. 70:1049-1057.
  • Seabra MC; Goldstein JL; Südhof TC; Brown MS. (15 Jul 1992). Rab geranylgeranyl transferase. A multisubunit enzyme that prenylates GTP-binding proteins terminating in Cys-X-Cys or Cys-Cys. J Biol Chem. 267:14497-14503.
  • Reiss Y; Seabra MC; Brown MS; Goldstein JL. (May 1992). p21ras farnesyltransferase: purification and properties of the enzyme. Biochem Soc Trans. 20:487-488.
  • Reiss Y; Seabra MC; Armstrong SA; Slaughter CA; Goldstein JL; Brown MS. (5 Jun 1991). Nonidentical subunits of p21H-ras farnesyltransferase. Peptide binding and farnesyl pyrophosphate carrier functions. J Biol Chem. 266:10672-10677.
  • Seabra MC; Reiss Y; Casey PJ; Brown MS; Goldstein JL. (3 May 1991). Protein farnesyltransferase and geranylgeranyltransferase share a common alpha subunit. Cell. 65:429-434.
  • Reiss Y; Goldstein JL; Seabra MC; Casey PJ; Brown MS. (13 Jul 1990). Inhibition of purified p21ras farnesyl:protein transferase by Cys-AAX tetrapeptides. Cell. 62:81-88.
  • Reiss, Y; Seabra, MC; Goldstein, JL; Brown, MS. (1990). Purification of ras Farnesyl:Protein Transferase. Meth Compan Meth Enzymol. 1:241-245.
  • Mesquita MF; Seabra MP; Halpern MJ. (May 1987). Simple carbohydrates in the diet. Am J Clin Nutr. 45:1197-1201.

Chapters in books

  • MacDonald I; Meltzer MR; Smaoui N. Choroideremia. In GeneReviews at GeneTests: Medical Genetics Information Resource [database online] 1997-2007. (Apr 2007). Author weblink.
  • van den Hurk, J.A.J.M.; Cremers, F.P.M.; Seabra, M.C.. CHM (Choroideremia) Gene. In Wiley Encylopedia of Molecular Medicine. 750-752. John Wiley & Sons Inc. (2002).
  • Seabra, M.C.. Biochemistry of Rab geranylgeranyl transferase. In The Enzymes. XXI:131-154. Tamanoi F (ed); Sigman D (ed). Academic Press, New York (2000).
  • Seabra, M.C.; Brown, M.S.; Goldstein, J.L.. Transferase, CAAX Geranylgeranyl Transferase, Rab Geranylgeranyl Transferase, Rab Escort Proteins (REP1 and REP2). In Guidebook to the Small GTPases. 41-48. Zerial M (ed); Huber L (ed). Oxford University Press (1995).

Conferences

  • Seabra MC. Rab GTPases in membrane traffic and disease. 33rd Annual Meeting of the German-Society-for-Cell-Biology/German Melanoma Research Network, 10 Mar 2010 - 13 Mar 2010. 89:32-32. ELSEVIER GMBH, URBAN & FISCHER VERLAG (1 Mar 2010).
  • Seabra MC. Pathogenesis of diseases involving defects in Rab GTPase function and membrane traffic. 36th FEBS Congress of the Biochemistry for Tomorrows Medicine, 25 Jun 2011 - 30 Jun 2011. 278:47-47. WILEY-BLACKWELL (1 Jun 2011).
  • Tavare R; Baron R; Taylor A; Seabra MC; Blazewska KM; McKenna CE; Kashemirov BA; Ebetino FH; et alRogers MJ; Coxon FP. Characterisation of the inhibition of rab prenylation by phosphonocarboxylates. International-Bone-and-Mineral-Society Davos Workshop on Bone Biology and Therapeutics, 9 Mar 2008 - 14 Mar 2008. 42:S88-S89. ELSEVIER SCIENCE INC (1 Mar 2008). DOI.
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