Methods Development: Novel Tools for High-Speed / High-Resolution optical imaging
Dr Gil Bub and Mr Peter Lee
A key aspect of imaging for bio-research is the drive towards combining high spatial and temporal resolution of structural and functional studies. To ‘square this circle’, one can take advantage of the fact that high pixel-count sensors are readily available on the consumer product market, by encoding time in the space domain. This has been achieved using fast pixel-accurate shuttering in a patented technique called Temporal Pixel Multiplexing (TPM). This is being implemented ‘on chip’ in collaboration with the team of Dr Mark Pitter at Nottingham University.
We are now applying TPM to several cardiac research questions. In addition, we are exploring the utility of light-emitting diodes, from ultraviolet to infrared, as alternative light sources for frame-accurate capture of fluorescent dye emission.
Funding: EPSRC, ISIS Innovation, and the Oxford Clarendon Fund.
Recent papers:
- Lee P, Bollensdorff C, Quinn TA, Wuskell JP, Loew LM & Kohl P. Single-sensor system for spatially-resolved, continuous, simultaneous and multi-parametric optical mapping of cardiac tissue. American Journal of Physiology (submitted).
- Bub G, Tecza M, Helmes M, Lee P & Kohl P. Temporal pixel multiplexing for simultaneous high-speed high-resolution imaging. Nature Methods 2010/7:209-211 (DOI).
