Publications

Wong SP, Argyros O, Howe SJ and Harbottle RP Systemic gene transfer of non-viral plasmids to neonatal mice Journal of Controlled Release (advance online publication December 2010; doi:10.1016/j.jconrel.2010.12.010)

Argyros O, Wong SP, Constantinos F, Tolmachov O, Waddington SN, Howe SJ, Niceta M, Coutelle C and Harbottle RP Development of S/MAR minicircles for enhanced and  persistent transgene expression in the mouse liver Journal of Molecular Medicine (Accepted for publication December 2010)

Al-Allaf FA, Coutelle C, Waddington SW, David A, Harbottle RP and Themis M LDLR-Gene therapy for familial hypercholesterolaemia: problems, progress, and perspectives International Archives of Medicine (advance online publication 2010, 3:36doi:10.1186/1755-7682-3-36)

Wong SP,  Argyros O, Coutelle C, and Harbottle RP Non-viral S/MAR vectors replicate episomally in vivo when provided with a selective advantage Gene Therapy (advance online publication 26 August 2010; doi: 10.1038/gt.2010.116)

Argyros O, Wong, SP, Harbottle RP,  Lipps HJ, Ogris M, Magnusson T, Pinto MGV, Haas J and Baiker A pEPito: a significantly improved non-viral episomal expression vector for mammalian cells. BMC biotechnology (2010) vol. 10 pp. 20

Wong SP, Argyros O, Coutelle C, Harbottle RP: Strategies for the episomal modification of cells. Curr Opin Mol Ther (2009) 11(4):433-441.

Coutelle C: Why bother? Is in utero gene therapy worth the effort? Mol Ther (2008) 16(2):219-220.

Buckley SMK, Waddington SN, Jezzard S, Bergau A, Themis M, MacVinish LJ, Cuthbert AW, Colledge WH, Coutelle C: Intra-amniotic delivery of CFTR-expressing adenovirus does not reverse cystic fibrosis phenotype in inbred CFTR-knockout mice. Mol Ther (2008) 16(5):819-824.

Buckley S, Howe S, Wong S, Buning H, McIntosh J, Baker A, Nathwani A, Thrasher A, Coutelle C, McKay T, Waddington S: Luciferase detection after intra-nasal vector delivery is improved by intra-nasal rather than intra-peritoneal luciferin administration. Hum Gene Ther (2008).

Buckley S, Howe S, Sheard V, Ward N, Coutelle C, Thrasher A, Waddington S, McKay T: Lentiviral transduction of the murine lung provides efficient pseudotype and developmental stage-dependent cell-specific transgene expression. Gene Therapy (2008).

 Argyros O, Wong S, Niceta M, Waddington S, Howe S, Coutelle C, Miller A, Harbottle RP, , Richard P: Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector. Gene Therapy (2008).

 Waddington SN, Buckley SM, David AL, Peebles DM, Rodeck CH, Coutelle C: Fetal gene transfer. Curr Opin Mol Ther (2007) 9(5):432-438.

 Tolmachov O, Coutelle C: Covalent attachment of multifunctional chimeric terminal proteins to 5' DNA ends: A potential new strategy for assembly of synthetic therapeutic gene vectors. Med Hypotheses (2007) 68(2):328-331.

Hibbitt OC, Harbottle RP, , Richard P, Waddington SN, Bursill CA, Coutelle C, Channon KM, Wade-Martins R: Delivery and long-term expression of a 135 kb LDLR genomic DNA locus in vivo by hydrodynamic tail vein injection. The journal of gene medicine (2007) 9(6):488-497.

Vaysse L, Gregory LG, Harbottle RP, , Richard P, Perouzel E, Tolmachov O, Coutelle C: Nuclear-targeted minicircle to enhance gene transfer with non-viral vectors in vitro and in vivo. The journal of gene medicine (2006) 8(6):754-763.

Tolmachov O, Palaszewski I, Bigger B, Coutelle C: RecET driven chromosomal gene targeting to generate a RecA deficient Escherichia coli strain for Cre mediated production of minicircle DNA. BMC Biotechnol (2006) 6:17.

Tolmachov O, Ma Y-L, Themis M, Patel P, Spohr H, Macleod KT, Ullrich ND, Kienast Y, Coutelle C, Peters NS: Overexpression of connexin 43 using a retroviral vector improves electrical coupling of skeletal myoblasts with cardiac myocytes in vitro. BMC cardiovascular disorders (2006) 6:25.

Guiller A, Bellido A, Coutelle A, Madec L: Spatial genetic pattern in the land mollusc Helix aspersa inferred from a 'centre-based clustering' procedure. Genet Res (2006) 88(1):27-44.

David AL, Weisz B, Gregory L, Themis M, Cook T, Roubliova X, Deprest J, Coutelle C, Rodeck CH, Peebles DM: Ultrasound-guided injection and occlusion of the trachea in fetal sheep. Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology (2006) 28(1):82-88.

David AL, Peebles DM, Gregory L, Waddington SN, Themis M, Weisz B, Ruthe A, Lawrence L, Cook T, Rodeck CH, Coutelle C: Clinically applicable procedure for gene delivery to fetal gut by ultrasound-guided gastric injection: toward prenatal prevention of early-onset intestinal diseases. Hum Gene Ther (2006) 17(7):767-779.

Bigger BW, Siapati EK, Mistry A, Waddington SN, Nivsarkar MS, Jacobs L, Perrett R, Holder MV, Ridler C, Kemball-Cook G, Ali RR et al: Permanent partial phenotypic correction and tolerance in a mouse model of hemophilia B by stem cell gene delivery of human factor IX. Gene Therapy (2006) 13(2):117-126.

Weisz B, David AL, Gregory LG, Perocheau D, Ruthe A, Waddington SN, Themis M, Cook T, Coutelle C, Rodeck CH, Peebles DM: Targeting the respiratory muscles of fetal sheep for prenatal gene therapy for Duchenne muscular dystrophy. Am J Obstet Gynecol (2005) 193(3 Pt 2):1105-1109.

Waterhouse JE, Harbottle RP, , Richard P, Keller M, Kostarelos K, Coutelle C, Jorgensen MR, Miller AD: Synthesis and application of integrin targeting lipopeptides in targeted gene delivery. Chembiochem (2005) 6(7):1212-1223.

Waddington SN, Kramer MG, Hernandez-Alcoceba R, Buckley SMK, Themis M, Coutelle C, Prieto J: In utero gene therapy: current challenges and perspectives. Mol Ther (2005) 11(5):661-676.

Tolmachov O, Harbottle RP, , Richard P, Bigger B, Coutelle C, Schleef M: Minimized, CpG-Depleted, and Methylated DNA Vectors: Toward Perfection in Nonviral Gene Therapy. DNA-Pharmaceuticals: Formulation and Delivery in Gene Therapy, DNA Vaccination and Immunotherapy (2005):43 - 52.

Themis M, Waddington SN, Schmidt M, von Kalle C, Wang Y, Al-Allaf F, Gregory LG, Nivsarkar M, Themis M, Holder MV, Buckley SMK et al: Oncogenesis following delivery of a nonprimate lentiviral gene therapy vector to fetal and neonatal mice. Mol Ther (2005) 12(4):763-771.

Hoare J, Waddington S, Thomas HC, Coutelle C, McGarvey MJ: Complement inhibition rescued mice allowing observation of transgene expression following intraportal delivery of baculovirus in mice. The journal of gene medicine (2005) 7(3):325-333.

Coutelle C, Themis M, Waddington SN, Buckley SMK, Gregory LG, Nivsarkar MS, David AL, Peebles D, Weisz B, Rodeck C: Gene therapy progress and prospects: fetal gene therapy--first proofs of concept--some adverse effects. Gene Therapy (2005) 12(22):1601-1607.

Buckley SMK, Waddington SN, Jezzard S, Lawrence L, Schneider H, Holder MV, Themis M, Coutelle C: Factors influencing adenovirus-mediated airway transduction in fetal mice. Mol Ther (2005) 12(3):484-492.

Al-Allaf FA, Tolmachov O, Themis M, Coutelle C: Coupled analysis of bacterial transformants and ligation mixture by duplex PCR enables detection of fatal instability of a nascent recombinant plasmid. J Biochem Biophys Methods (2005) 64(2):142-146.

Al-Allaf F, Tolmachov O, Themis M, Coutelle C: Accurate size gauging of ExoIII/S1-generated deletions by PCR analysis of ligation mixtures. Anal Biochem (2005) 339(2):348-350.

Waddington SN, Nivsarkar MS, Mistry AR, Buckley SMK, Kemball-Cook G, Mosley KL, Mitrophanous K, Radcliffe P, Holder MV, Brittan M, Georgiadis A et al: Permanent phenotypic correction of hemophilia B in immunocompetent mice by prenatal gene therapy. Blood (2004) 104(9):2714-2721.

Vaysse L, Harbottle RP, , Richard P, Bigger B, Bergau A, Tolmachov O, Coutelle C: Development of a self-assembling nuclear targeting vector system based on the tetracycline repressor protein. J Biol Chem (2004) 279(7):5555-5564.

Keller M, Harbottle RP, , Richard P, Perouzel E, Colin M, Shah I, Rahim A, Vaysse L, Bergau A, Moritz S, Brahimi-Horn C, Coutelle C et al: Nuclear localisation sequence templated nonviral gene delivery vectors: investigation of intracellular trafficking events of LMD and LD vector systems. Chembiochem (2003) 4(4):286-298.

Gregory LG, Harbottle RP, , Richard P, Lawrence L, Knapton HJ, Themis M, Coutelle C: Enhancement of adenovirus-mediated gene transfer to the airways by DEAE dextran and sodium caprate in vivo. Mol Ther (2003) 7(1):19-26.

Tagawa T, Manvell M, Brown N, Keller M, Perouzel E, Murray KD, Harbottle RP, , Richard P, Tecle M, Booy F, Brahimi-Horn MC, Coutelle C et al: Characterisation of LMD virus-like nanoparticles self-assembled from cationic liposomes, adenovirus core peptide mu and plasmid DNA. Gene Ther (2002) 9(9):564-576.

Jost PJ, Harbottle RP, , Richard P, Knight A, Miller AD, Coutelle C, Schneider H: A novel peptide, THALWHT, for the targeting of human airway epithelia. FEBS Lett (2001) 489(2-3):263-269.

Colin M, Moritz S, Fortanges P, Kornprobst M, Delouis C, Keller M, Miller AD, Capeau J, Coutelle C, Brahimi-Horn.M.C: The nuclear pore complex is involved in nuclear transfer of plasmid DNA condensed with an oligolysine-RGD peptide containing nuclar localisation properties. Gene Therapy (2001) 8:1643-1653.

Bigger B, Tolmachov O, Collombet J, Fragkos M, Palaszewski I, Coutelle C: An araC-controlled bacterial cre expression system to produce DNA minicircle vectors for nuclear and mitochondrial gene therapy. J Biol Chem (2001) 276(25):23018-23027.

Colin M, Maurice M, Trugnan G, Kornprobst M, Harbottle RP, , Richard P, Knight A, Cooper RG, Miller AD, Capeau J, Coutelle C, Brahimi-Horn MC: Cell delivery, intracellular trafficking and expression of an integrin-mediated gene transfer vector in tracheal epithelial cells. Gene Ther (2000) 7(2):139-152.

Villaverde A, Feliu JX, Arís A, Harbottle RP, , Richard P, Benito A, Coutelle C: A cell adhesion peptide from foot-and-mouth disease virus can direct cell targeted delivery of a functional enzyme. Biotechnol Bioeng (1999) 59(3):294-301.

Cooper R, Harbottle RP, , Richard P, Schneider H, Coutelle C: Peptide Mini-Vectors for Gene Delivery. Angewandte Chemie International Edition (1999).

Colin M, Harbottle RP, , Richard P, Knight A, Kornprobst M, Cooper RG, Miller AD, Trugnan G, Capeau J, Coutelle C, Brahimi-Horn MC: Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells. Gene Ther (1999) 5(11):1488-1498.

Schneider H, Harbottle RP, , Richard P, Yokosaki Y, Kunde J, Sheppard D, Coutelle C: A novel peptide, PLAEIDGIELTY, for the targeting of alpha9beta1-integrins. FEBS Lett (1998) 429(3):269-273.

Hart SL, Arancibia-Cárcamo CV, Wolfert MA, Mailhos C, O'Reilly NJ, Ali RR, Coutelle C, George AJT, Harbottle RP, , Richard P, Knight AM, Larkin DFP et al: Lipid-mediated enhancement of transfection by a non-viral integrin-targeting vector. H Gene Ther (1998) 9:575-585.

Hart SL, Arancibia-Cárcamo CV, Wolfert MA, Mailhos C, O'Reilly NJ, Ali RR, Coutelle C, George AJ, Harbottle RP, , Richard P, Knight AM, Larkin DF et al: Lipid-mediated enhancement of transfection by a nonviral integrin-targeting vector. Hum Gene Ther (1998) 9(4):575-585.

Harbottle RP, , Richard P, Cooper RG, Hart SL, Ladhoff A, McKay T, Knight AM, Wagner E, Miller AD, Coutelle C: An RGD-oligolysine peptide: a prototype construct for integrin-mediated gene delivery. Hum Gene Ther (1998) 9(7):1037-1047.

Colin M, Harbottle RP, , Richard P, Knight A, Kornprobst M, Cooper R, Miller A, Trugnan G, Capeau J, Coutelle C, Brahimi-Horn M: Liposomes enhance delivery and expression of an RGD-oligolysine gene transfer vector in human tracheal cells. Gene Ther (1998) 5(11):1488-1498.

Mistry AR, Falciola L, Monaco L, Tagliabue R, Acerbis G, Knight A, Harbottle RP, , Richard P, Soria M, Bianchi ME, Coutelle C, Hart SL: Recombinant HMG1 protein produced in Pichia pastoris: a nonviral gene delivery agent. BioTechniques (1997) 22(4):718-729.

Villaverde A, Feliu JX, Harbottle RP, , Richard P, Benito A, Coutelle C: A recombinant, arginine-glycine-aspartic acid (RGD) motif from foot-and-mouth disease virus binds mammalian cells through vitronectin and, to a lower extent, fibronectin receptors. Gene (1996) 180(1-2):101-106.

Hart SL, Harbottle RP, , Richard P, Cooper R, Miller A, Williamson R, Coutelle C: Gene delivery and expression mediated by an integrin-binding peptide. Gene Ther (1995) 2(8):552-554.

Caplen NJ, Alton EW, Middleton PG, Dorin JR, Stevenson BJ, Gao X, Durham SR, Jeffery PK, Hodson ME, Coutelle C: Liposome-mediated CFTR gene transfer to the nasal epithelium of patients with cystic fibrosis. Nat Med (1995) 1(1):39-46.

Hart SL, Knight AM, Harbottle RP, , Richard P, Mistry A, Hunger H-D, Cutler DF, Williamson R, Coutelle C: Cell binding and internalization by filamentous phage displaying a cyclic Arg-Gly-Asp-containing peptide. JBiolChem (1994) 269:12468-12474.