Cell Sorting

The facility provides high quality cell-sorting services

Sort scheduling is done by contacting the facility directly

In order to allocate an appropriate amount of time for your sort, please have the following information regarding your sort ready:

• % of positive cells need to be sorted (if known)
• Number of cells/samples and cell type
• Fluorochromes or dyes being used (please consult us before purchasing your antibodies)
• Type of collection (tube, plate, dish, etc…)
• Whether or not the sort needs to remain sterile.

 For cell sorting charges please click Charges

Sorting protocol:

A. Cell Preparation for cell sorting:

•  Prepare your cells as you normally would for flow cytometry analysis. (please consult us before purchasing your antibodies)
•  To set up the machine, you need to provide 0.5 to 1x 10⁵ cells/ml non-stained (negative control) cells in 1-2 ml volume.
• Provide 0.5 to 1x 10⁵ cells/ml of single stained cells or comp beads to set up compensation. 
•  For sorting, labeled cells should be at 5x 10⁶ per ml in FACS Tubes (BD cat number BD2058) in PBS (with no FCS).
• It is essential to filter the cells through a cell strainer immediately before sorting. We provide autoclavable filter mesh.

B. Collection tubes:

2 way or 4 way sorting: Cells are sorted into 15mL Falcon tubes containing 0.5ml of 10% FCS in Media (with antibiotics). We can also sort into 96-well plates, microscope slides or dishes.

 C. Policies

• If you do not have the proper controls for your sort, no guarantee is made regarding the outcome of the sort.  
• Sort start times and end times will be strictly enforced barring any instrument malfunction. 
• If you cancel less than 24 hours in advance, you will be charged for 50% of the time you reserved. 
• Failure to inform us of a cancellation, or “no shows” will be charged the full amount for the time booked.