Services and Charges
We provide:
- Training and cell sorting plus experimental design.
- Teaching theoretical and practical aspects of flow cytometry, MSD and Luminex technologies are provided together with help in designing experiments and analysis.
- We also have a number of standalone analysis computers that can be used for off-line analysis.
Training Procedure:
Training is mandatory for all first-time users of the facility; this is regardless of previous flow cytometry experience.
- Fill in and submit the user database form.
- Arrange for a meeting with us to discuss/design your experiment.
- Schedule the first training session. 3 training sessions, 6 hours in total.
- After your training is complete you will be provided with a users account on the instrument.
- Then you will be given permission to access our online calendar.
- To access the FACS facility on evenings/weekends, we will to arrange for your swipe card to be activated. If you are from outside Imperial, a temporary swipe card will be issued.
Our facility provides:
- BD LSRII Fortessa - 18 colours
- BD FACS LSR II - 15 colours
- BD FACS Canto II - 8 colours plus HTS loader
- CyAn™ ADP LX - 9 colours
- CyAn™ ADP LX - 9 colours
- Sony ICyt Eclipse
- BD FAcs Aria IIIU (Upgraded High Speed cell sorter) - 9 colours
- Luminex 100
- Bio-Rad Bio-Plex 200
- MSD machine
The hourly charges for the usage of the machines:
Machine |
Price |
FACS analysers |
£25 |
Luminex Xmap |
£25 |
FACS sorter |
£50* |
* Sort setup: please add 1 hour for non sterile sorting and 2 hours for sterile sorting.
Charges are calculated based on the booking done on the calendar (the start time of the reservation until the end of the reservation) this is compared to the log in book to calculate the time spent or booked the machine for.
Your Files/Data
FACS users are responsible for their own files.
After acquisition copy your files IMMEDIATELY onto the X drive (ask us about this), the data can be burned onto CD on your computer. It might be wise to do 2 CDs, so that one can be at work, the other at home, in case of a fire. Your files on the machines will be deleted every month (the 2nd working day of each month). So make sure you ALWAYS backup IMMEDIATELY after sample acquisition.
