Professor Ana Pombo

Publications


Journals

  • Brookes E; Pombo A. (1 Apr 2012). Code breaking: The RNAPII modification code in pluripotency. Cell Cycle. 11:1267-1268. DOI.
  • Brookes E; de Santiago I; Hebenstreit D; Morris KJ; Carroll T; Xie SQ; Stock JK; Heidemann M; et alEick D; Nozaki N; Kimura H; Ragoussis J; Teichmann SA; Pombo A. (3 Feb 2012). Polycomb Associates Genome-wide with a Specific RNA Polymerase II Variant, and Regulates Metabolic Genes in ESCs. Cell Stem Cell. 10:157-170. DOI.
  • Pombo A; Starr DA. (15 Mar 2011). Nuclear cell biology. Mol Biol Cell. 22:722. DOI.
  • Chotalia M; Pombo A. (Mar 2011). Polycomb targets seek closest neighbours. PLoS Genet. 7:e1002031. DOI.
  • Alder O; Lavial F; Helness A; Brookes E; Pinho S; Chandrashekran A; Arnaud P; Pombo A; et alO'Neill L; Azuara V. (1 Aug 2010). Ring1B and Suv39h1 delineate distinct chromatin states at bivalent genes during early mouse lineage commitment. Development. 137:2483-2492. DOI.
  • Kanhere A; Viiri K; Araujo CC; Rasaiyaah J; Bouwman RD; Whyte WA; Pereira CF; Brookes E; et alWalker K; Bell GW; Pombo A; Fisher AG; Young RA; Jenner RG. none N (ed). (11 Jun 2010). Short RNAs are transcribed from repressed polycomb target genes and interact with polycomb repressive complex-2. Molecular Cell. 38:675-688. Publisher weblink DOI.
  • Pombo A; Gilbert DM. (Jun 2010). Nucleus and gene expression: the structure and function conundrum. Curr Opin Cell Biol. 22:269-270. DOI.
  • Ferrai C; de Castro IJ; Lavitas L; Chotalia M; Pombo A. (Jun 2010). Gene positioning. Cold Spring Harb Perspect Biol. 2:a000588. DOI.
  • Landeira D; Sauer S; Poot R; Dvorkina M; Mazzarella L; Jørgensen HF; Pereira CF; Leleu M; et alPiccolo FM; Spivakov M; Brookes E; Pombo A; Fisher C; Skarnes WC; Snoek T; Bezstarosti K; Demmers J; Klose RJ; Casanova M; Tavares L; Brockdorff N; Merkenschlager M; Fisher AG. (Jun 2010). Jarid2 is a PRC2 component in embryonic stem cells required for multi-lineage differentiation and recruitment of PRC1 and RNA Polymerase II to developmental regulators. Nat Cell Biol. 12:618-624. DOI.
  • Ferrai C; Xie SQ; Luraghi P; Munari D; Ramirez F; Branco MR; Pombo A; Crippa MP. (5 Jan 2010). Poised transcription factories prime silent uPA gene prior to activation. PLoS Biology. 8:e1000270. DOI.
  • Xie SQ; Lavitas LM; Pombo A. (2010). CryoFISH: fluorescence in situ hybridization on ultrathin cryosections. Methods Mol Biol. 659:219-230. DOI.
  • Morris KJ; Chotalia M; Pombo A. (2010). Nuclear architecture in stem cells. Adv Exp Med Biol. 695:14-25. DOI.
  • Brookes E; Pombo A. (Nov 2009). Modifications of RNA polymerase II are pivotal in regulating gene expression states. EMBO Rep. 10:1213-1219. DOI.
  • Baddeley D; Chagin VO; Schermelleh L; Martin S; Pombo A; Carlton PM; Gahl A; Domaing P; et alBirk U; Leonhardt H; Cremer C; Cardoso MC. (Jan 2010). Measurement of replication structures at the nanometer scale using super-resolution light microscopy. Nucleic Acids Res. 38:e8. DOI.
  • Ferrai C; Pombo A. (Jan 2009). 3D chromatin regulation of Sonic hedgehog in the limb buds. Dev Cell. 16:9-11. DOI.
  • Maya-Mendoza A; Tang CW; Pombo A; Jackson DA. none (ed). (1 Jan 2009). Mechanisms regulating S phase progression in mammalian cells. Frontiers in Bioscience. 14:4199-4213.
  • Hiragami-Hamada K; Xie SQ; Saveliev A; Uribe-Lewis S; Pombo A; Festenstein R. (2009). The molecular basis for stability of heterochromatin-mediated silencing in mammals. Epigenetics Chromatin. 2:14. DOI.
  • Babu MM; Janga SC; de Santiago I; Pombo A. Teichmann S (ed); Patel N (ed). (Dec 2008). Eukaryotic gene regulation in three dimensions and its impact on genome evolution. Current Opinion in Genetics and Development. 18:571-582. DOI.
  • Noordermeer D; Branco MR; Splinter E; Klous P; van Ijcken W; Swagemakers S; Koutsourakis M; van der Spek P; et alPombo A; de Laat W. none (ed). (7 Mar 2008). Transcription and chromatin organization of a housekeeping gene cluster containing an integrated beta-globin locus control region. PLoS Genetics. 4:e1000016. DOI.
  • Branco MR; Branco T; Ramirez F; Pombo A. (2008). Changes in chromosome organization during PHA-activation of resting human lymphocytes measured by cryo-FISH. Chromosome Res. 16:413-426. DOI.
  • Stock JK; Giadrossi S; Casanova M; Brookes E; Vidal M; Koseki H; Brockdorff N; Fisher AG; et alPombo A. (Dec 2007). Ring1-mediated ubiquitination of H2A restrains poised RNA polymerase II at bivalent genes in mouse ES cells. Nat Cell Biol. 9:1428-1435. DOI.
  • Pombo A; Branco MR. (Oct 2007). Functional organisation of the genome during interphase. Curr Opin Genet Dev. 17:451-455. DOI.
  • Pombo A. (Aug 2007). Advances in imaging the interphase nucleus using thin cryosections. Histochem Cell Biol. 128:97-104. DOI.
  • Piercy RJ; Zhou H; Feng L; Pombo A; Muntoni F; Brown SC. (Apr 2007). Desmin immunolocalisation in autosomal dominant Emery-Dreifuss muscular dystrophy. Neuromuscul Disord. 17:297-305. DOI.
  • Branco MR; Pombo A. (Mar 2007). Chromosome organization: new facts, new models. Trends Cell Biol. 17:127-134. DOI.
  • Branco MR; Pombo A. (May 2006). Intermingling of chromosome territories in interphase suggests role in translocations and transcription-dependent associations. PLoS Biol. 4:e138. DOI.
  • Xie SQ; Martin S; Guillot PV; Bentley DL; Pombo A. (Apr 2006). Splicing speckles are not reservoirs of RNA polymerase II, but contain an inactive form, phosphorylated on serine2 residues of the C-terminal domain. Mol Biol Cell. 17:1723-1733. DOI.
  • Xie SQ; Pombo A. (Jan 2006). Distribution of different phosphorylated forms of RNA polymerase II in relation to Cajal and PML bodies in human cells: an ultrastructural study. Histochem Cell Biol. 125:21-31. DOI.
  • Chow CM; Georgiou A; Szutorisz H; Maia e Silva A; Pombo A; Barahona I; Dargelos E; Canzonetta C; et alDillon N. (Apr 2005). Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division. EMBO Rep. 6:354-360. DOI.
  • Kenny E; Mason D; Saoudi A; Pombo A; Ramírez F. (May 2004). CD8 alpha is an activation marker for a subset of peripheral CD4 T cells. Eur J Immunol. 34:1262-1271. DOI.
  • Guillot PV; Xie SQ; Hollinshead M; Pombo A. (1 May 2004). Fixation-induced redistribution of hyperphosphorylated RNA polymerase II in the nucleus of human cells. Exp Cell Res. 295:460-468. DOI.
  • Martin S; Failla AV; Spöri U; Cremer C; Pombo A. (May 2004). Measuring the size of biological nanostructures with spatially modulated illumination microscopy. Mol Biol Cell. 15:2449-2455. DOI.
  • Boyd DC; Pombo A; Murphy S. (2 Oct 2003). Interaction of proteins with promoter elements of the human U2 snRNA genes in vivo. Gene. 315:103-112.
  • Pombo A. (Jan 2003). Cellular genomics: which genes are transcribed, when and where?. Trends Biochem Sci. 28:6-9.
  • Martin S; Pombo A. (2003). Transcription factories: quantitative studies of nanostructures in the mammalian nucleus. Chromosome Res. 11:461-470.
  • Politz J; Pombo A. (2002). Genomics meets nanoscience: probing genes and the cell nucleus at 10-9 meters. Genome Biol. 3:REPORTS4007.
  • Kenny E; Mason D; Pombo A; Ramírez F. (Oct 2000). Phenotypic analysis of peripheral CD4+ CD8+ T cells in the rat. Immunology. 101:178-184.
  • Jackson DA; Pombo A; Iborra F. (Feb 2000). The balance sheet for transcription: an analysis of nuclear RNA metabolism in mammalian cells. FASEB J. 14:242-254.
  • Pombo A; Jones E; Iborra FJ; Kimura H; Sugaya K; Cook PR; Jackson DA. (2000). Specialized transcription factories within mammalian nuclei. Crit Rev Eukaryot Gene Expr. 10:21-29.
  • Pombo A; Jackson DA; Hollinshead M; Wang Z; Roeder RG; Cook PR. (15 Apr 1999). Regional specialization in human nuclei: visualization of discrete sites of transcription by RNA polymerase III. EMBO J. 18:2241-2253. DOI.
  • Pombo A; Hollinshead M; Cook PR. (Apr 1999). Bridging the resolution gap: Imaging the same transcription factories in cryosections by light and electron microscopy. J Histochem Cytochem. 47:471-480.
  • Wykes M; Pombo A; Jenkins C; MacPherson GG. (1 Aug 1998). Dendritic cells interact directly with naive B lymphocytes to transfer antigen and initiate class switching in a primary T-dependent response. J Immunol. 161:1313-1319.
  • Jackson DA; Pombo A. (23 Mar 1998). Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S phase in human cells. J Cell Biol. 140:1285-1295.
  • Pombo A; Cuello P; Schul W; Yoon JB; Roeder RG; Cook PR; Murphy S. (16 Mar 1998). Regional and temporal specialization in the nucleus: a transcriptionally-active nuclear domain rich in PTF, Oct1 and PIKA antigens associates with specific chromosomes early in the cell cycle. EMBO J. 17:1768-1778. DOI.
  • Pombo A; Cook PR. (15 Dec 1996). The localization of sites containing nascent RNA and splicing factors. Exp Cell Res. 229:201-203. DOI.
  • Iborra FJ; Pombo A; McManus J; Jackson DA; Cook PR. (15 Dec 1996). The topology of transcription by immobilized polymerases. Exp Cell Res. 229:167-173. DOI.
  • Iborra FJ; Pombo A; Jackson DA; Cook PR. (Jun 1996). Active RNA polymerases are localized within discrete transcription "factories' in human nuclei. J Cell Sci. 109 ( Pt 6):1427-1436.
  • Hughes TA; Pombo A; McManus J; Hozák P; Jackson DA; Cook PR. (1995). On the structure of replication and transcription factories. J Cell Sci Suppl. 19:59-65.
  • Pombo A; Ferreira J; Bridge E; Carmo-Fonseca M. (1 Nov 1994). Adenovirus replication and transcription sites are spatially separated in the nucleus of infected cells. EMBO J. 13:5075-5085.

Chapters in books

  • Guillot PV; Martin S; Pombo A. The organization of transcription in the nucleus of mammalian cells. 95-105. Hemmerich P (ed); Diekmann S (ed). American Scientific Publ., CA, USA (2005).
  • Branco MR; Xie SQ; Martin S; Pombo A. Correlative microscopy using Tokuyasu cryosections: applications for immunogold labelling and in situ hybridisation. 201-207. Scion Publishing Ltd, Bloxham, UK (2006).

Conferences

  • Pombo A; Jackson DA; Iborra FJ; Hollinshead M; Kimura H; Sugaya K; Cook PR. Transcription factories. 12th Eur Congr. on Electron Microscopy, 2000. Volume I Biological Sciences:B461-B464. (2000).
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